daoy human medulloblastoma cell line  (ATCC)

Journal: bioRxiv

Article Title: Ca 2+ -mediated protein citrullination regulates proliferation in the regenerating and malignant CNS

doi: 10.64898/2026.01.25.701650

Figure Lengend Snippet: (A) Normalised FLUO-4 fluorescence traces (F/F₀) in DAOY cells, showing normal activity (0–6 min) and reduced signal following BAPTA-AM treatment (6–12 min). (B–C) Maximum-intensity projections of FLUO-4 fluorescence over 2 min windows in untreated (B; 2–4 min) and BAPTA-AM–treated (C; 8–10 min) cells. (D) Quantification of average calcium signal (F/F₀) across time windows shown in (B–C).

Article Snippet: DAOY medulloblastoma cells (ATCC, Cat. No. HTB-186) were maintained in Minimum Essential Medium Eagle (EMEM; Sigma-Aldrich, Cat. No. M0325) supplemented with 10% fetal bovine serum (FBS).

Techniques: Fluorescence, Activity Assay

Journal: bioRxiv

Article Title: Ca 2+ -mediated protein citrullination regulates proliferation in the regenerating and malignant CNS

doi: 10.64898/2026.01.25.701650

Figure Lengend Snippet: (A–D) Representative EdU incorporation assays (green) after 24 h treatment with DMSO (A, B) or BB-Cl-amidine (C, D; 6 μM). DAPI (magenta). Scale bars, 500 μm (A, B) and 100 μm (C, D). (E) Quantification of EdU incorporation in DAOY cells treated with BB-Cl-amidine or DMSO. (F) Growth-curve analysis of DAOY cell proliferation over 120 h following BB-Cl-amidine or DMSO treatment. (G–H) Representative maximum-intensity images of DAOY cells showing citrullinated histone H3 immunoreactivity after 24 h treatment with DMSO (G) or BB-Cl-amidine (H; 6 μM). Scale bars, 50 μm. (I) Quantification of average H4Cit3 intensity in (G) and (H). (J) Xenotransplantation experimental overview. (K) Side view of DiI -labelled DAOY cell pellets (red) transplanted into the midbrain parenchyma of 2 dpf embryos. Scale bar, 1 mm. (L) Magnified view of (K). Scale bar, 200 μm. (M) Imaging strategy for tracking xenograft volume over time. (N–W) Top-down maximum-intensity projections of fluorescently labelled DAOY xenografts from 0 to 4 dpi in untreated embryos (N–R) and embryos treated with BB-Cl-amidine (S–W; 40 μM). (X) Quantification of normalised tumour volume following DMSO or BB-Cl-amidine treatment.

Article Snippet: DAOY medulloblastoma cells (ATCC, Cat. No. HTB-186) were maintained in Minimum Essential Medium Eagle (EMEM; Sigma-Aldrich, Cat. No. M0325) supplemented with 10% fetal bovine serum (FBS).

Techniques: Imaging

Journal: bioRxiv

Article Title: Ca 2+ -mediated protein citrullination regulates proliferation in the regenerating and malignant CNS

doi: 10.64898/2026.01.25.701650

Figure Lengend Snippet: (A–C) Flow cytometry plots showing propidium iodide (PI) versus Hoechst fluorescence in DAOY cells treated with 0.1% DMSO (A), 6 μM BB-Cl-amidine (B), or 0.1% Triton X (C, positive control). (D) Quantification of PI-labelled cells from conditions shown in (A–C).

Article Snippet: DAOY medulloblastoma cells (ATCC, Cat. No. HTB-186) were maintained in Minimum Essential Medium Eagle (EMEM; Sigma-Aldrich, Cat. No. M0325) supplemented with 10% fetal bovine serum (FBS).

Techniques: Flow Cytometry, Fluorescence, Positive Control

Journal: bioRxiv

Article Title: Ca 2+ -mediated protein citrullination regulates proliferation in the regenerating and malignant CNS

doi: 10.64898/2026.01.25.701650

Figure Lengend Snippet: (A–C) Flow cytometry plots showing Annexin V–FITC versus propidium iodide (PI) staining in DAOY cells treated with 0.1% DMSO (A), 6 μM BB-Cl-amidine (B), or 2 μM staurosporine (C, positive control). (D) Quantification of early apoptotic cells (Annexin V⁺/PI⁻) from conditions shown in (A–C).

Article Snippet: DAOY medulloblastoma cells (ATCC, Cat. No. HTB-186) were maintained in Minimum Essential Medium Eagle (EMEM; Sigma-Aldrich, Cat. No. M0325) supplemented with 10% fetal bovine serum (FBS).

Techniques: Flow Cytometry, Staining, Positive Control

Journal: Cancers

Article Title: Exploring Novel Applications: Repositioning Clinically Approved Therapies for Medulloblastoma Treatment

doi: 10.3390/cancers17223659

Figure Lengend Snippet: Relevance of targeting BCL2 for the treatment of medulloblastoma. ( A ) Box-dot plot showing mRNA expression level of BCL2 (R2: Genomics Analysis and Visualization Platform ( http://r2.amc.nl accessed on 10 November 2025) in normal cerebellum ( C ) ( n = 9 retrieved from Roth database), and medulloblastoma patients (M1–M7); M1: ( n = 76) from Gilbertson database, M2: ( n = 57) from Delattre database, M3: ( n = 62) from Kool database, M4: ( n = 223) from Pfister1 database, M5: ( n = 51) from den Boer database, M6: ( n = 31) from Hsieh database and M7: ( n = 73) from Pfister2 database. Statistical analysis (One-way analysis of variance (ANOVA)) is shown, p = 7.64 × 10 −19 . ( B ) Immunoblot analysis of BCL2 expression in HMC3 (H), ONS76 (O), DAOY (D), and HDMB03 (HD) cells. HSP90 was used as a loading control. Densitometric quantification of BCL2 expression is shown, normalized to HSP90 levels (BCL2/HSP90). Original western blots are presented in . ( C – F ) Dose-dependent inhibition of viability of the four cells lines by increasing concentrations of venetoclax. *: p < 0.05; **: p < 0.01; ***: p < 0.001: **** p < 0.0001; NS: Non Significant. ( G ) IC 50 and specificity index of Venetoclax for the different cell lines.

Article Snippet: The human medulloblastoma cell lines (ONS76, DAOY, HD-MB03) and the normal microglial cells (HMC3) were purchased from American Type Culture Collection (ATCC).

Techniques: Expressing, Western Blot, Control, Inhibition

Journal: ACS Bio & Med Chem Au

Article Title: Synthesis of a Water-Soluble BODIPY for Targeting and Assessing the Function of Endoplasmic Reticulum

doi: 10.1021/acsbiomedchemau.5c00142

Figure Lengend Snippet: Viability of CCD18Co-htert and DAOY cells assessed using MTT 48 h after incubation with Tris-BODIPY-OH 1 at different concentrations (0–10 μM). The results are shown as mean ± SEM of three biological replicates. One-way ANOVA was used to measure significance (comparison to the 0 μM condition).

Article Snippet: Human medulloblastoma DAOY cells (ATCC HTB-186) were grown in DMEM supplemented with 10% FCS and penicillin/streptomycin antibiotics.

Techniques: Incubation, Comparison

Journal: Cancer Research

Article Title: De Novo Serine Synthesis Is a Metabolic Vulnerability That Can Be Exploited to Overcome Sunitinib Resistance in Advanced Renal Cell Carcinoma

doi: 10.1158/0008-5472.CAN-24-1393

Figure Lengend Snippet: Sunitinib treatment enhances de novo serine metabolism across multiple cancer models. A, Immunoblots various cancer lines, including 786-O (ccRCC), BT549 (TNBC), A549 (lung), DAOY (medulloblastoma), and Cal33 (head and neck), treated with DMSO (veh) or sunitinib (sun; 2.5 μmol/L) for 48 hours. B, Cell count measurements conducted 96 hours after treating BT549, A549, DAOY, and Cal33 with DMSO (veh), sunitinib (2.5 μmol/L), NCT-503 (NCT; 30 μmol/L), or a combination of both treatments. Three different experiments were conducted. ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-way ANOVA).

Article Snippet: Human medulloblastoma cell line Daoy (HTB-186, RRID: CVCL_1167) was purchased from the ATCC and maintained in minimum essential medium with GlutaMAX (Thermo Fisher Scientific, Montigny-le-Bretonneux) supplemented with 10% FBS and 1 mmol/L sodium pyruvate.

Techniques: Western Blot, Cell Counting